Association of Variant rs7903146(c/t) Single Nucleotide Polymorphism of Transcription Factor 7-like 2 Gene with Newly Detected Hyperglycemia in Pregnancy

Asha Ranjan; Neeraj Kumar Agrawal; Snehil Budhwar; Varsha Ranjan

doi:10.4103/ijem.ijem_511_21

Association of Variant rs7903146(c/t) Single Nucleotide Polymorphism of Transcription Factor 7-like 2 Gene with Newly Detected Hyperglycemia in Pregnancy

Indian J Endocrinol Metab. 2022 Mar-Apr;26(2):154-159. doi: 10.4103/ijem.ijem_511_21. Epub 2022 Apr 29.

Authors

Asha Ranjan¹, Neeraj Kumar Agrawal¹, Snehil Budhwar², Varsha Ranjan³

Affiliations

¹ Department of Endocrinology and Metabolism, IMS, BHU, Varanasi, UP, India.
² Department of Human and Molecular Genetics, Institute of Science, BHU, Varanasi, UP, India.
³ Research Officer, ICMR, National Institute of Medical Statistics, New Delhi, India.

Abstract

Background: Transcription factor 7-like 2 (TCF7L2) gene has a significant role in hyperglycemia in pregnancy (HIP) risk. The current study was planned with the aim to evaluate the association of single nucleotide polymorphism (SNP) rs7903146 in patients of newly detected HIP among Indian population of northern region.

Methods: This study was an observational case control study done among newly detected HIP (The World Health Organization (WHO) criteria, 2013) and healthy pregnant females without diabetes. Participants from both the group were genotyped for rs7903146 (C/T) variant of TCF7L2 gene using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.

Results: A total of 71 cases of newly detected HIP were included in the study, out of which 25 (35.2%) of them were of first-time detected diabetes mellitus in pregnancy (DIP) and 46 (64.7%) were of gestational diabetes (GDM) and 100 were pregnant females without diabetes in third trimester were enrolled as controls. Average age of participants in the case group was 28.7 ± 4.0 years and the control group were 26.5 ± 3.6 years (P value 0.09). The wild homozygous CC genotype, heterozygous CT genotype and homozygous TT genotype were present in 39.4%, 53.5%, 7.1% of case group vs 53%, 43% and 4% of control group, respectively. No significant association of rs7903146(C/T) SNP of TCF7L2 gene in HIP (CC/CT, CC/TT P value 0.15, 0.38, respectively) in our population was found. There was no significant difference in the distribution of genotypes between DIP and GDM.

Conclusion: This study shows no evidence of association of rs7903146(C/T) SNP of TCF7L2 gene with newly detected HIP in our population.

Keywords: Gestational diabetes mellitus; hyperglycemia; pregnancy.