Structure of VAT, a CDC48/p97 ATPase homologue from the archaeon Thermoplasma acidophilum as studied by electron tomography

FEBS Lett. 1999 May 14;451(1):27-32. doi: 10.1016/s0014-5793(99)00431-7.

Abstract

Valosine-containing protein-like ATPase from Thermoplasma acidophilum is a member of the superfamily of ATPases associated with a diversity of cellular activities and is closely related to CDC48 from yeast and p97 from higher eukaryotes and more distantly to N-ethylmaleimide-sensitive fusion protein. We have used electron tomography to obtain low-resolution (2-2.5 nm) three-dimensional maps of both the whole 500 kDa complex and the N-terminally truncated valosine-containing protein-like ATPase from T. acidophilum complex lacking the putative substrate binding domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / ultrastructure*
  • Archaeal Proteins
  • Cell Cycle Proteins
  • Microscopy, Electron
  • Thermoplasma / enzymology*
  • Valosin Containing Protein

Substances

  • Archaeal Proteins
  • Cell Cycle Proteins
  • Adenosine Triphosphatases
  • Valosin Containing Protein
  • vat protein, Thermoplasma acidophilum

Associated data

  • PDB/UNKNOWN