Abstract
We have developed a strategy for improving the stimulation of T cells during immune responses by constructing recombinant antibodies that enhance the delivery of antigen to antigen-presenting cells, such as B cells. These antibodies have variable regions specific for surface molecules on B cells, and a constant region with an inserted antigen. In vitro, such antibodies make B cells approximately 1000-fold more efficient at presenting antigen and stimulating specific T cells. In vivo, the antibodies turn B cells of the spleen into potent stimulators of T cells. This approach may be useful for the generation of new vaccines.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies / genetics
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Antibodies / immunology*
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Antibody Specificity
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Antigen Presentation
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B-Lymphocytes / immunology*
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Epitopes / immunology*
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Genes, Immunoglobulin
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Histocompatibility Antigens Class II / immunology
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Immunoglobulin D / immunology*
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Immunoglobulin D / metabolism
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Immunoglobulin Variable Region / genetics
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Lymphocyte Activation
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Mice
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Mice, Inbred BALB C
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Mice, SCID
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Mice, Transgenic
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Molecular Sequence Data
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Protein Engineering*
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Recombinant Proteins / immunology
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T-Lymphocytes / immunology*
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Vaccination
Substances
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Antibodies
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Epitopes
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Histocompatibility Antigens Class II
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Immunoglobulin D
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Immunoglobulin Variable Region
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Recombinant Proteins
Associated data
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GENBANK/AF072827
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GENBANK/AF072828