Abstract
Systemic acquired resistance (SAR) is a plant immune response induced after a local infection by necrotizing pathogens. The Arabidopsis NPR1 gene is a positive regulator of SAR, essential for transducing the SAR signal salicylic acid (SA). Mutations in the NPR1 gene abolish the SA-induced expression of pathogenesis-related (PR) genes and resistance to pathogens. To identify additional regulators of SAR, we screened for suppressors of npr1-1. In the npr1-1 background, the sni1 (suppressor of npr1-1, inducible 1) mutant shows near wild-type levels of PR1 expression and resistance to pathogens after induction. Restoration of SAR in npr1-1 by the recessive sni1 mutation indicates that wild-type SNI1 may function as a negative regulator of SAR. We cloned the SNI1 gene and found that it encodes a leucine-rich nuclear protein.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Arabidopsis / genetics
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Arabidopsis / microbiology
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Arabidopsis / physiology*
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Arabidopsis Proteins*
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Base Sequence
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Chromosome Mapping
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Cloning, Molecular
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DNA, Complementary
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Gene Expression Regulation, Plant*
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Genes, Plant
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Genes, Suppressor
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Glucuronidase / genetics
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Mice
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Nuclear Proteins / genetics
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Nuclear Proteins / physiology*
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Plant Proteins / chemistry
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Plant Proteins / genetics*
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Plant Proteins / metabolism*
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Plants, Genetically Modified
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Pseudomonas / pathogenicity
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Recombinant Fusion Proteins / biosynthesis
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Retinoblastoma Protein / chemistry
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Retinoblastoma Protein / genetics
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Sequence Alignment
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Sequence Homology, Amino Acid
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Signal Transduction
Substances
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Arabidopsis Proteins
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DNA, Complementary
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NPR1 protein, Arabidopsis
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Nuclear Proteins
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Plant Proteins
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Recombinant Fusion Proteins
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Retinoblastoma Protein
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SNI1 protein, Arabidopsis
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Glucuronidase