Fluorescent automated single-stranded conformation (F-SSCP) analysis is able to detect a point mutation at the extreme 5' end of a PCR product

Clin Biochem. 1999 Aug;32(6):481-4. doi: 10.1016/s0009-9120(99)00046-6.

Authors

F J Pirie¹, D F York, A A Motala, M A Omar

Affiliation

¹ Department of Medicine, University of Natal, Durban, South Africa. pirie@med.und.ac.za

PMID: 10667486
DOI: 10.1016/s0009-9120(99)00046-6

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Mutational Analysis / methods*
Deoxyribonucleases, Type II Site-Specific / genetics
Deoxyribonucleases, Type II Site-Specific / metabolism
Fluorescence
Humans
Point Mutation*
Polymerase Chain Reaction / methods*
Polymorphism, Single-Stranded Conformational*
Sensitivity and Specificity

Substances

Deoxyribonucleases, Type II Site-Specific
GATC-specific type II deoxyribonucleases