Ferroverdins, inhibitors of cholesteryl ester transfer protein produced by Streptomyces sp. WK-5344. I. Production, isolation and biological properties

H Tomoda; N Tabata; M Shinose; Y Takahashi; H B Woodruff; S Omura

doi:10.7164/antibiotics.52.1101

Ferroverdins, inhibitors of cholesteryl ester transfer protein produced by Streptomyces sp. WK-5344. I. Production, isolation and biological properties

J Antibiot (Tokyo). 1999 Dec;52(12):1101-7. doi: 10.7164/antibiotics.52.1101.

Authors

H Tomoda¹, N Tabata, M Shinose, Y Takahashi, H B Woodruff, S Omura

Affiliation

¹ Research Center for Biological Function, The Kitasato Institute and Graduate School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.

PMID: 10695673
DOI: 10.7164/antibiotics.52.1101

Abstract

Streptomyces sp. WK-5344, a soil isolate, was found to produce structurally related inhibitors of cholesteryl ester transfer protein (CETP). New active compounds, designated ferroverdins B and C, were isolated along with known ferroverdin A from the fermentation broth by solvent extraction, ODS column chromatography and silica gel column chromatography. All ferroverdins showed a dose-dependent inhibitory activity against human CETP. The IC50 values were 21, 0.62 and 2.2 microM for ferroverdins A, B and C, respectively, indicating that ferroverdin B is one of the most potent CETP inhibitors of microbial origin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / drug effects
Carrier Proteins / antagonists & inhibitors*
Cholesterol Ester Transfer Proteins
Dose-Response Relationship, Drug
Fermentation
Ferrous Compounds / isolation & purification*
Ferrous Compounds / pharmacology
Glycoproteins*
Humans
Nitroso Compounds / isolation & purification*
Nitroso Compounds / pharmacology
Streptomyces / classification
Streptomyces / metabolism*

Substances

CETP protein, human
Carrier Proteins
Cholesterol Ester Transfer Proteins
Ferrous Compounds
Glycoproteins
Nitroso Compounds
ferroverdin B
ferroverdin C
ferroverdin