The genetic analysis of F1 hybrid larvae between female Trichinella spiralis and male Trichinella britovi

Parasitol Int. 2000 Feb;48(4):289-95. doi: 10.1016/s1383-5769(99)00031-8.

Abstract

Hybrids between female Trichinella spiralis and male Trichinella britovi were constructed. Then, hybrid genotype was characterized by DNA markers including mitochondrial cytochrome c oxidase subunit I (CO I) gene, the gene encoding the 43-kDa excretory-secretory (ES) protein, and genomic DNA fragments specific for T. spiralis and T. britovi identified from random amplified polymorphism DNA (RAPD). PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial CO I gene revealed that all hybrids carried a T. spiralis pattern. The same analysis of the gene encoding the 43-kDa ES protein showed that each hybrid carried both T. spiralis and T. britovi gene type simultaneously. In the analysis of genomic DNA using RAPD-derived PCR primers, some hybrids carried T. spiralis and T. britovi-specific RAPD markers, while others carried the RAPD marker of T. spiralis only.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Helminth*
  • Crosses, Genetic*
  • DNA Primers / chemistry
  • DNA, Helminth / chemistry
  • DNA, Helminth / genetics
  • Electron Transport Complex IV / chemistry
  • Electron Transport Complex IV / genetics
  • Female
  • Genotype
  • Helminth Proteins / chemistry
  • Helminth Proteins / genetics
  • Larva / genetics
  • Male
  • Mice
  • Polymorphism, Restriction Fragment Length
  • Random Amplified Polymorphic DNA Technique
  • Trichinella / chemistry
  • Trichinella / genetics*
  • Trichinella spiralis / chemistry
  • Trichinella spiralis / genetics*
  • Trichinellosis / genetics
  • Trichinellosis / parasitology

Substances

  • Antigens, Helminth
  • DNA Primers
  • DNA, Helminth
  • Helminth Proteins
  • excretory-secretory antigen, Trichinella
  • Electron Transport Complex IV