Abstract
In order to achieve an EPR sensitive probe for DNA, 3-carboxy-Proxyl free radical was linked to O-6 of dG through a five-atoms-tether. The modified base was incorporated into a 30-mer ODN, then annealed to its complementary DNA strand. Hydrodynamic parameters show only a slight destabilization with respect to the equivalent unlabeled hybrid. EPR could monitor the hybrid formation showing a progressive enlargement of the upfield signal in passing from the labeled ss- to the ds-30-mer.
MeSH terms
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Cyclic N-Oxides / chemistry*
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DNA / analysis*
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Deoxyguanosine / analogs & derivatives
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Deoxyguanosine / chemical synthesis*
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Deoxyguanosine / chemistry
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Electron Spin Resonance Spectroscopy
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Fluorescence Polarization
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Free Radicals
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Nucleic Acid Conformation
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Organophosphorus Compounds / chemical synthesis*
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Organophosphorus Compounds / chemistry
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Spectrophotometry, Ultraviolet
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Spin Labels / chemical synthesis*
Substances
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5'-O-(4,4'-dimethoxytrityl)-O(6)-(3-(2,2,5,5-tetramethyl-1-pyrrolidinyloxy-3-carboxamido)propyl)deoxyguanosine-3'-(O-cyanoethyl-N,N-diisopropyl)phosphoramidite
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Cyclic N-Oxides
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Free Radicals
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Organophosphorus Compounds
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Spin Labels
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2,2,5,5-tetramethyl-1-pyrrolidinyloxy-3-carboxylic acid
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DNA
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Deoxyguanosine