The MAK-V protein kinase regulates endocytosis in mouse

Mol Gen Genet. 2000 Nov;264(4):411-8. doi: 10.1007/s004380000293.

Abstract

We report the cloning of a mouse cDNA encoding the MAK-V protein kinase, with a putative specificity for serine/threonine residues. The mak-v gene is transcribed in adult brain and in the mouse embryo from at least 7.5 dpc. Using the yeast two-hybrid system, we showed that MAK-V interacts with Rabaptin-5, a protein which plays an important role in endocytosis. Functional studies of the MAK-V protein suggest that it regulates endocytosis. We also constructed a human mak-v cDNA and localized the human mak-v gene at 21q22.11. Its chromosomal location suggests that mak-v could be involved in disorders of the nervous system, development or in malignancies.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain / enzymology
  • Chromosomes, Human, Pair 21 / genetics
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • Endocytosis / physiology*
  • Gene Expression
  • Humans
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue / metabolism
  • Protein Kinases / genetics*
  • Protein Kinases / physiology*
  • Protein Serine-Threonine Kinases / genetics*
  • Protein Serine-Threonine Kinases / physiology*
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Two-Hybrid System Techniques

Substances

  • DNA Primers
  • DNA, Complementary
  • Protein Kinases
  • Hunk protein, mouse
  • Protein Serine-Threonine Kinases