Abstract
We report the cloning of a mouse cDNA encoding the MAK-V protein kinase, with a putative specificity for serine/threonine residues. The mak-v gene is transcribed in adult brain and in the mouse embryo from at least 7.5 dpc. Using the yeast two-hybrid system, we showed that MAK-V interacts with Rabaptin-5, a protein which plays an important role in endocytosis. Functional studies of the MAK-V protein suggest that it regulates endocytosis. We also constructed a human mak-v cDNA and localized the human mak-v gene at 21q22.11. Its chromosomal location suggests that mak-v could be involved in disorders of the nervous system, development or in malignancies.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Brain / enzymology
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Chromosomes, Human, Pair 21 / genetics
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Cloning, Molecular
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DNA Primers / genetics
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DNA, Complementary / genetics
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Endocytosis / physiology*
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Gene Expression
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Humans
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Mice
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Molecular Sequence Data
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Nerve Tissue / metabolism
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Protein Kinases / genetics*
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Protein Kinases / physiology*
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Protein Serine-Threonine Kinases / genetics*
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Protein Serine-Threonine Kinases / physiology*
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Sequence Homology, Amino Acid
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Species Specificity
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Two-Hybrid System Techniques
Substances
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DNA Primers
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DNA, Complementary
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Protein Kinases
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Hunk protein, mouse
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Protein Serine-Threonine Kinases