Atomic resolution structure of a mutant of the spectrin SH3 domain

Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):337-40. doi: 10.1107/s090744490001581x.

Abstract

The crystal structure of an alpha-spectrin Src-homology 3 (SH3) domain mutant has been refined at 1.12 A resolution. This X-ray structure is at the highest resolution achieved so far for an SH3 domain. The structure allows the identification of a complete set of specific interactions and is useful for the elucidation of relations between structure and pH-dependent thermodynamic stability in a series of SH3 domain mutants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crystallization
  • Crystallography, X-Ray
  • Hydrogen Bonding
  • Models, Molecular
  • Mutation
  • Protein Structure, Secondary
  • Sensitivity and Specificity
  • Spectrin / chemistry*
  • Spectrin / genetics
  • Spectrin / isolation & purification
  • src Homology Domains*

Substances

  • Spectrin

Associated data

  • PDB/1G2B