Objective: To investigate the mechanism of Jianpi Yiqi Recipe (JPYQR) in inhibition of gastric mucosa injury.
Methods: A rat model of acute injury of gastric mucosa was established by binding and immersing in water. Ulcer index (UI) was measured, change of heat shock protein (HSP70) in plasma and gastric tissue was examined, and the gastric HSPs was studied immunohistochemically.
Results: The UI in rats pretreated with JPYQR or Lizhudele was lower than that of model group (P < 0.001), and UI of JPYQR group was lower than that of Lizhudele group (P < 0.01). On the other hand, the HSP70 level in the two pretreated groups were significantly higher than that of the model group (P < 0.05), and the plasma HSP70 level of JPYQR group was higher than that of Lizhudele group (P < 0.05). The difference of gastric HSP70 level between the two pretreated groups was insignificant (P > 0.05). Immunohistochemical study revealed a high level expression of HSP70 and HSP60 by glands and the level of the expression in model group was the highest, followed by those in Lizhudele group and JPYQR group.
Conclusion: JPYQR could enhance the expression of HSPs in rats, and so as to prevent the gastric mucosa from injury. HSPs might involve in the defending mechanism of gastric mucosa.