Echoviruses 1 and 8 are closely related genetically, and bind to similar determinants within the VLA-2 I domain

Virus Res. 2001 Jul;76(1):1-8. doi: 10.1016/s0168-1702(01)00226-x.

Abstract

Echoviruses (EV) 1 and 8 were originally considered to be distinct serotypes, but more recently have been considered strains of the same virus. In experiments with chimeric recombinant fusion proteins, both viruses bound to the I domain of the integrin VLA-2, and both required the same receptor residues for attachment. A full-length, infectious cDNA clone encoding EV1 was obtained; its nucleotide sequence was determined, as were the sequences encoding the EV8 capsid. EV1 and 8 show 94% amino acid identity within the capsid region and are more similar to each other than to any other human picornavirus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Capsid / genetics
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA, Recombinant / genetics
  • Enterovirus B, Human / chemistry
  • Enterovirus B, Human / genetics*
  • Enterovirus B, Human / metabolism*
  • HeLa Cells
  • Humans
  • Mice
  • Models, Molecular
  • Molecular Sequence Data
  • Phylogeny
  • Protein Binding
  • Protein Structure, Tertiary
  • Receptors, Very Late Antigen / chemistry*
  • Receptors, Very Late Antigen / genetics
  • Receptors, Very Late Antigen / metabolism*
  • Receptors, Virus / chemistry*
  • Receptors, Virus / genetics
  • Receptors, Virus / metabolism*
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • DNA, Recombinant
  • Receptors, Very Late Antigen
  • Receptors, Virus
  • Recombinant Fusion Proteins