Abstract
Echoviruses (EV) 1 and 8 were originally considered to be distinct serotypes, but more recently have been considered strains of the same virus. In experiments with chimeric recombinant fusion proteins, both viruses bound to the I domain of the integrin VLA-2, and both required the same receptor residues for attachment. A full-length, infectious cDNA clone encoding EV1 was obtained; its nucleotide sequence was determined, as were the sequences encoding the EV8 capsid. EV1 and 8 show 94% amino acid identity within the capsid region and are more similar to each other than to any other human picornavirus.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Binding Sites
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Capsid / genetics
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Cloning, Molecular
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DNA, Complementary / genetics
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DNA, Recombinant / genetics
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Enterovirus B, Human / chemistry
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Enterovirus B, Human / genetics*
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Enterovirus B, Human / metabolism*
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HeLa Cells
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Humans
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Mice
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Models, Molecular
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Molecular Sequence Data
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Phylogeny
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Protein Binding
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Protein Structure, Tertiary
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Receptors, Very Late Antigen / chemistry*
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Receptors, Very Late Antigen / genetics
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Receptors, Very Late Antigen / metabolism*
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Receptors, Virus / chemistry*
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Receptors, Virus / genetics
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Receptors, Virus / metabolism*
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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DNA, Recombinant
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Receptors, Very Late Antigen
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Receptors, Virus
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Recombinant Fusion Proteins