Synthesis, purification and biodistribution of (205)Bi-DOTMP, visualizing bone deposition patterns with autoradiography

Nucl Med Biol. 2001 May;28(4):425-33. doi: 10.1016/s0969-8051(01)00202-5.

Abstract

A HPLC system has been developed for carrier free and rapid delivery in a physiological buffer of the alpha-particle emitting bone-seeking radiopharmaceutical (212)Bi-DOTMP. (205)Bi-DOTMP was synthesized and HPLC purified to mimic and visualize the deposition pattern in bony tissues of (212)Bi-DOTMP by autoradiography. Inhomogeneous bone deposits were found with the highest concentration in the bone matrix, the endosteum and in the growth zones of young mice. Analysis of urine samples showed that (205)Bi-DOTMP was cleared as an intact complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoradiography
  • Bismuth / chemistry*
  • Bone and Bones / diagnostic imaging*
  • Bone and Bones / metabolism*
  • Chromatography, High Pressure Liquid
  • Female
  • Mice
  • Mice, Nude
  • Organophosphorus Compounds / chemical synthesis*
  • Organophosphorus Compounds / isolation & purification
  • Organophosphorus Compounds / pharmacokinetics*
  • Radioisotopes
  • Radionuclide Imaging
  • Radiopharmaceuticals / chemical synthesis*
  • Radiopharmaceuticals / isolation & purification
  • Radiopharmaceuticals / pharmacokinetics*
  • Tissue Distribution

Substances

  • Organophosphorus Compounds
  • Radioisotopes
  • Radiopharmaceuticals
  • 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(methylenephosphonic acid)
  • Bismuth