Use of real-time quantitative PCR to detect Chlamydophila felis infection

C Helps; N Reeves; S Tasker; D Harbour

doi:10.1128/JCM.39.7.2675-2676.2001

Use of real-time quantitative PCR to detect Chlamydophila felis infection

J Clin Microbiol. 2001 Jul;39(7):2675-6. doi: 10.1128/JCM.39.7.2675-2676.2001.

Authors

C Helps¹, N Reeves, S Tasker, D Harbour

Affiliation

¹ Division of Molecular and Cellular Biology, Department of Clinical Veterinary Science, University of Bristol, Langford, Bristol, BS40 5DU, United Kingdom. c.r.helps@Bristol.ac.uk

Abstract

A real-time PCR assay was developed to detect and quantify Chlamydophila felis infection of cats. The assay uses a molecular beacon to specifically identify the major outer membrane protein gene, is highly reproducible, and is able to detect fewer than 10 genomic copies.

Publication types

Evaluation Study

MeSH terms

Animals
Cat Diseases / diagnosis*
Cat Diseases / microbiology
Cats
Chlamydophila / genetics
Chlamydophila / isolation & purification*
Chlamydophila Infections / diagnosis
Chlamydophila Infections / microbiology
Chlamydophila Infections / veterinary*
Conjunctiva / microbiology
Conjunctivitis, Bacterial / diagnosis
Conjunctivitis, Bacterial / microbiology
Conjunctivitis, Bacterial / veterinary
DNA, Bacterial / analysis
Polymerase Chain Reaction / methods*
Reproducibility of Results

Substances

DNA, Bacterial