In recent years microsatellite marker applications in various disciplines increased rapidly. Microsatellites are characterized as independent and highly variable markers most noted for their usefulness and popularity among researchers. Microsatellites can be constructed in-house from a partial genomic library, ordered from a vendor, or "borrowed" from a species that is closely related to the target species. Microsatellite application in natural populations can reveal present as well as historical impacts of stressors to particular population.