Fluorescence resonance energy transfer dye nucleotide terminators: a new synthetic approach for high-throughout DNA sequencing

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):361-7. doi: 10.1081/NCN-100002307.

Abstract

Fluorescence resonance energy transfer (FRET) based dye-nucleotide terminators (10-13) were designed, synthesized, and formulated with Thermo Sequenase II DNA polymerase into a robust kit for high throughput DNA sequencing. The key energy transfer (ET) rigid and linear linker (2), required for the syntheses of energy transfer cassettes (6-9) was synthesized via Heck coupling reaction on t-Boc-L-4-iodo-phenylalanine (1) with N-TFA-propargylamine.

MeSH terms

  • Base Sequence
  • DNA / analysis
  • Energy Transfer
  • Fluorescence
  • Fluorescent Dyes / chemical synthesis*
  • Molecular Sequence Data
  • Nucleotides / chemical synthesis*
  • Sequence Analysis, DNA / methods*
  • Spectrometry, Fluorescence / methods

Substances

  • Fluorescent Dyes
  • Nucleotides
  • DNA