Four color fret dye nucleotide terminators for DNA sequencing

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):673-6. doi: 10.1081/NCN-100002348.

Abstract

The synthesis of four color set of energy transfer-dye terminators (8a-8d) starting from p-iodo-beta-phenylalanine was accomplished and their utility in the sequencing reactions has been evaluated.

MeSH terms

  • Energy Transfer
  • Fluorescent Dyes / chemical synthesis*
  • Phenylalanine / analogs & derivatives*
  • Phenylalanine / chemistry*
  • Sequence Analysis, DNA / methods*
  • Stereoisomerism

Substances

  • Fluorescent Dyes
  • Phenylalanine
  • 4-iodophenylalanine