A recombinant inbred population of soybean was developed from a intraspecies cross of Kefeng1 and Nannong 1138-2. A genetic linkage group of soybean genome was constructed, which composed of 22 linkage groups, included 302 loci, covered 2,363.8 cM. The interval mapping was used to identify quantitative trait loci (QLTs) associated with ten important agronomic traits in the RIL population. The results indicated that thirty-four QTLs were detected for the ten traits associated with yield and seed quality. We conclude that QTL analysis offers a useful tool for the genetic improvement of soybean cultivars.