A field experiment from June 1983 to October 1997 was conducted to study the effect of long-term no-tillage and application of manure on soil fertility properties. The results showed that the contents of soil organic C, total N and available N in the treatment of fourteen consecutive year no-tillage were all significantly higher in 0-5 cm soil layer, while, lower in 5-10 and 10-20 cm layers than in conventional tillage. The content of water stable aggregate in 0-20 cm layer was increased in no-tillage in that the soil structure was less destroyed. Based on various soil fertility properties, the contribution order of different fertilization treatments to soil fertility was as follows: pig manure > straw > green manure > chemical fertilizer > no fertilizer.