[The use of the two-hybrid cloning in yeast for functional characterization of protein kinase MAK-V]

I V Korobko; E V Korobko; N I Chupikova; M A Vanechkin; E V Smirnova; S L Kiselev; G P Georgiev

[The use of the two-hybrid cloning in yeast for functional characterization of protein kinase MAK-V]

Mol Biol (Mosk). 2002 May-Jun;36(3):491-5.

[Article in Russian]

Authors

I V Korobko¹, E V Korobko, N I Chupikova, M A Vanechkin, E V Smirnova, S L Kiselev, G P Georgiev

Affiliation

¹ Institute of Gene Biology, Russian Academy of Sciences, Moscow, 117334 Russia.

PMID: 12068635

Abstract

Identification of interaction partners opens a way to direct functional characterization of proteins. Several cDNAs coding for potential partners of protein kinase MAK-V/Hunk were isolated using two-hybrid cloning in yeast. Based on the partner properties, MAK-V/Hunk was assumed to play a role in tumorigenesis and tumor progression. With the previous results of two-hybrid cloning, MAK-V/Hunk was shown to participate in vesicular transport.

MeSH terms

Cadherins*
Cloning, Molecular
GTPase-Activating Proteins / genetics
GTPase-Activating Proteins / metabolism
Membrane Proteins / genetics
Membrane Proteins / metabolism
Protein Kinases / genetics*
Protein Kinases / metabolism*
Protein Serine-Threonine Kinases
Two-Hybrid System Techniques*
Vesicular Transport Proteins*
Yeasts / genetics*

Substances

Cadherins
E6-target protein 1
GTPase-Activating Proteins
Membrane Proteins
Rabep1 protein, mouse
Vesicular Transport Proteins
fat1 protein, mouse
Protein Kinases
Hunk protein, mouse
Protein Serine-Threonine Kinases