Tissue tagging can be implemented during cardiovascular magnetic resonance imaging exams to assist with the quantification of left ventriculargeometry, volume and ejection fraction, endocardial thickening and relaxation, and myocardial stress-strain relationships. During tagged cine gradient echo image acquisitions of left ventricular wall motion, rows of k-space data can be acquired with various phase-encoding orders, and the reconstruction of supplemental images can be accomplished using a variety of interpolation techniques. In this study, we investigated the utility of various phase order and segment interpolation methods for determining accurate tag displacement trajectories. Center-out phase order image acquisition with reconstruction using linear interpolation provided the highest tag position and displacement accuracy. Therefore, it is recommended that myocardial tagging exams be acquired with center-out phase encode order and reconstructed using linear segment interpolation when used for performing quantitative analysis of cardiovascular structure and function.