Use of the sensititre colorimetric microdilution panel for antifungal susceptibility testing of dermatophytes

J Clin Microbiol. 2002 Jul;40(7):2618-21. doi: 10.1128/JCM.40.7.2618-2621.2002.

Abstract

The Sensititre YeastOne antifungal panel was used to test 49 dermatophytes belonging to the species Epidermophyton floccosum, Microsporum gypseum, Microsporum canis, Trichophyton tonsurans, Trichophyton rubrum, and Trichophyton mentagrophytes. The MICs of four antifungals obtained with the Sensititre YeastOne antifungal panel were compared with those obtained by the reference NCCLS microdilution method. The levels of agreement between the two methods (<or=2 dilutions) were 81.6% with amphotericin B, 87.7% with itraconazole, 67.3% with fluconazole, and 69.4% with ketoconazole.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amphotericin B / pharmacology
  • Antifungal Agents / pharmacology
  • Arthrodermataceae / drug effects*
  • Colorimetry
  • Coloring Agents
  • Dermatomycoses / drug therapy
  • Dermatomycoses / microbiology
  • Drug Resistance, Fungal
  • Epidermophyton / drug effects
  • Fluconazole / pharmacology
  • Humans
  • Itraconazole / pharmacology
  • Ketoconazole / pharmacology
  • Microbial Sensitivity Tests / methods*
  • Microbial Sensitivity Tests / statistics & numerical data
  • Microsporum / drug effects
  • Oxazines*
  • Sensitivity and Specificity
  • Trichophyton / drug effects
  • Xanthenes*

Substances

  • Antifungal Agents
  • Coloring Agents
  • Oxazines
  • Xanthenes
  • resazurin
  • Itraconazole
  • Amphotericin B
  • Fluconazole
  • Ketoconazole