Photosensitizing effect of protoporphyrin IX in pigmented melanoma of mice

Biochem Biophys Res Commun. 2002 Sep 27;297(3):468-72. doi: 10.1016/s0006-291x(02)02238-6.

Abstract

No fluorescence of protoporphyrin IX (PpIX) was measured using a fiber optic probe in pigmented B16F10 melanoma in mice after topical application of 5-aminolevulinic acid methylester (ALA-Me). However, chemical extraction of tissues excised from mice after intratumoral administration of ALA-Me or its parent compound ALA revealed that this tumor had the capability to produce PpIX. Small amounts of endogenous porphyrins, mainly PpIX, were found in the melanoma not treated with these drugs. Topical application of ALA-Me followed by exposure with laser light (633nm) delayed the growth of the tumors slightly. Light alone also had a significant effect on the tumor growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Topical
  • Alamethicin / therapeutic use
  • Aminolevulinic Acid / administration & dosage
  • Aminolevulinic Acid / analogs & derivatives*
  • Aminolevulinic Acid / therapeutic use*
  • Animals
  • Cell Division / drug effects
  • Hutchinson's Melanotic Freckle / drug therapy
  • Hutchinson's Melanotic Freckle / pathology
  • Melanoma, Experimental / drug therapy*
  • Melanoma, Experimental / pathology
  • Mice
  • Photosensitizing Agents / administration & dosage
  • Photosensitizing Agents / therapeutic use*
  • Protoporphyrins / administration & dosage
  • Protoporphyrins / chemistry
  • Protoporphyrins / therapeutic use*
  • Skin Neoplasms / drug therapy*
  • Skin Neoplasms / pathology
  • Spectrometry, Fluorescence
  • Tumor Cells, Cultured

Substances

  • Photosensitizing Agents
  • Protoporphyrins
  • delta-aminolevulinic acid methyl ester
  • Alamethicin
  • Aminolevulinic Acid
  • protoporphyrin IX