Melting curve analysis of feline calicivirus isolates detected by real-time reverse transcription PCR

Chris Helps; Philippa Lait; Séverine Tasker; Dave Harbour

doi:10.1016/s0166-0934(02)00167-2

Melting curve analysis of feline calicivirus isolates detected by real-time reverse transcription PCR

J Virol Methods. 2002 Dec;106(2):241-4. doi: 10.1016/s0166-0934(02)00167-2.

Authors

Chris Helps¹, Philippa Lait, Séverine Tasker, Dave Harbour

Affiliation

¹ Division of Molecular and Cellular Biology, Department of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol BS40 5DU, UK. c.r.helps@bristol.ac.uk

PMID: 12393155
DOI: 10.1016/s0166-0934(02)00167-2

Abstract

A real-time reverse transcription polymerase chain reaction assay was developed to detect feline calicivirus infection in cats. Using SYBR green I melting curve analysis, isolates could be distinguished by their melting temperature. The assay is sensitive, linear over a wide range of template concentrations and allows accurate quantitation of viral load.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Caliciviridae Infections / diagnosis
Caliciviridae Infections / veterinary*
Caliciviridae Infections / virology
Calicivirus, Feline / genetics
Calicivirus, Feline / isolation & purification*
Cat Diseases / diagnosis*
Cat Diseases / virology
Cats
Nucleic Acid Denaturation
Reverse Transcriptase Polymerase Chain Reaction / methods*
Reverse Transcriptase Polymerase Chain Reaction / veterinary