Abstract
The EVI1 gene in chromosome band 3q26 exhibits a number of properties consistent with a role as an oncogene, and its expression is activated in most myeloid leukemia patients with, as well as in a minority of patients without, 3q26 rearrangements. A splice variant of this gene, MDS1/EVI1, acts as its antagonist at least in some tissue culture assays. We established real-time quantitative reverse transcriptase polymerase chain reaction (RTQ-RT-PCR) assays for these mRNA variants to compare their expression levels in a quantitatively reliable manner. EVI1 was overexpressed to highly variable extents in all patients with, as well as in 14% of patients without, 3q26 rearrangements. In some of these samples, MDS1/EVI1 was also transcribed at elevated levels compared to those of healthy controls. However, although the induction of MDS1/EVI1 was comparable to, or higher than, that of EVI1 in three of five samples with a normal EVI1 locus, this was true for only two of 13 patients with a 3q26 aberration. We further provide preliminary evidence that the RTQ-RT-PCR assay may be useful for disease monitoring in patients overexpressing EVI1.
Copyright 2002 Wiley-Liss, Inc.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adult
-
Aged
-
Alternative Splicing / genetics
-
Child
-
Chromosome Aberrations
-
Chromosome Banding
-
Chromosomes, Human, Pair 3 / genetics
-
Cyclophilins / genetics
-
DNA-Binding Proteins / antagonists & inhibitors*
-
DNA-Binding Proteins / biosynthesis*
-
DNA-Binding Proteins / genetics
-
Disease Progression
-
Female
-
Humans
-
K562 Cells
-
Leukemia, Myeloid / genetics
-
Leukemia, Myeloid / metabolism*
-
Leukemia, Myeloid / pathology
-
MDS1 and EVI1 Complex Locus Protein
-
Male
-
Middle Aged
-
Molecular Sequence Data
-
Oncogene Proteins, Fusion*
-
Proto-Oncogenes*
-
Recombinant Fusion Proteins / biosynthesis*
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / physiology*
-
Reverse Transcriptase Polymerase Chain Reaction / methods
-
Transcription Factors / antagonists & inhibitors
-
Transcription Factors / biosynthesis
-
Transcription Factors / genetics
-
Tumor Cells, Cultured
-
U937 Cells
Substances
-
DNA-Binding Proteins
-
MDS1 and EVI1 Complex Locus Protein
-
MDS1-EVI1 fusion protein, human
-
MECOM protein, human
-
Oncogene Proteins, Fusion
-
Recombinant Fusion Proteins
-
Transcription Factors
-
Cyclophilins
Associated data
-
GENBANK/AF487422
-
GENBANK/AF487423
-
GENBANK/AF487424