Molecular cloning, mRNA expression and chromosomal localization of mouse angiotensin-converting enzyme-related carboxypeptidase (mACE2)

DNA Seq. 2002 Aug;13(4):217-20. doi: 10.1080/1042517021000021608.

Abstract

We isolated two mouse cDNA clones which show significant similarities with human angiotensin-converting enzyme-related carboxypeptidase (ACE2). The cDNAs were 2746 and 1995 bp in length and seemed to arise from the same gene by alternative splicing. The longer cDNA encoded a 798-amino acid protein containing the sequence motif conserved among zinc metallopeptidases. Mouse ACE2 showed 83% identity with human ACE2. Northern blot analysis revealed that 2.8- and 2.0-kb transcripts were expressed mainly in the kidney and the lungs. The mouse ACE2 gene was mapped to chromosome X 70.5 cM.

MeSH terms

  • Amino Acid Sequence
  • Angiotensin-Converting Enzyme 2
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Carboxypeptidases / biosynthesis
  • Carboxypeptidases / genetics*
  • Chromosome Mapping
  • Chromosomes, Human, X*
  • Cloning, Molecular
  • Gene Expression Regulation, Enzymologic
  • Humans
  • Kidney
  • Lung
  • Mice
  • Molecular Sequence Data
  • Open Reading Frames
  • Peptidyl-Dipeptidase A
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Tissue Distribution

Substances

  • Carboxypeptidases
  • Peptidyl-Dipeptidase A
  • ACE2 protein, human
  • Ace2 protein, mouse
  • Angiotensin-Converting Enzyme 2

Associated data

  • GENBANK/AB053181
  • GENBANK/AB053182