A cell-based bar code reader for high-throughput screening of ion channel-ligand interactions

Anal Chem. 2002 Dec 15;74(24):6133-8. doi: 10.1021/ac026133f.

Abstract

This paper presents a microfluidics-patch clamp platform for performing high-throughput screening and rapid characterization of weak-affinity ion channel-ligand interactions. This platform integrates a microfluidic chip consisting of multiple channels entering an open volume with standard patch clamp equipment. The microfluidic chip is placed on a motorized scanning stage and the method relies on the ability to scan rapidly, on the order of milliseconds, a patch-clamped cell across discrete zones of different solutions created in the open volume. Under ideal conditions, this method has the capacity to obtain kinetically resolved patch clamp measurements and dose-response curves of up to 10(3) ligand solutions in a single day.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods
  • Cell Line
  • Dose-Response Relationship, Drug
  • Electronic Data Processing*
  • Ion Channels / metabolism*
  • Ligands
  • Microchemistry / instrumentation
  • Microchemistry / methods
  • Patch-Clamp Techniques / instrumentation
  • Patch-Clamp Techniques / methods

Substances

  • Ion Channels
  • Ligands