Use of real-time PCR to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" DNA

Séverine Tasker; Chris R Helps; Michael J Day; Tim J Gruffydd-Jones; Dave A Harbour

doi:10.1128/JCM.41.1.439-441.2003

Use of real-time PCR to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" DNA

J Clin Microbiol. 2003 Jan;41(1):439-41. doi: 10.1128/JCM.41.1.439-441.2003.

Authors

Séverine Tasker¹, Chris R Helps, Michael J Day, Tim J Gruffydd-Jones, Dave A Harbour

Affiliation

¹ Department of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol, BS40 5DU, United Kingdom. s.tasker@bristol.ac.uk

Abstract

A real-time PCR assay using Taqman probes was developed to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" in feline blood samples. The assay was rapid and sensitive and was successfully used to monitor the in vivo kinetics of cats experimentally infected with each species.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cat Diseases / microbiology
Cats
DNA, Bacterial / analysis
Disease Models, Animal
Kinetics
Mycoplasma / classification
Mycoplasma / genetics
Mycoplasma / isolation & purification*
Mycoplasma Infections / microbiology*
Mycoplasma Infections / veterinary
Polymerase Chain Reaction / methods
Sensitivity and Specificity

Substances

DNA, Bacterial