Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit

J Clin Microbiol. 2003 Feb;41(2):826-30. doi: 10.1128/JCM.41.2.826-830.2003.

Abstract

We have shown that dermatophyte species can be easily identified on the basis of a DNA sequence encoding a part of the large-subunit (LSU) rRNA (28S rRNA) by using the MicroSeq D2 LSU rRNA Fungal Sequencing Kit. Two taxa causing distinct dermatophytoses were clearly distinguished among isolates of the Trichophyton mentagrophytes species complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arthrodermataceae / isolation & purification*
  • Base Sequence
  • Cats
  • DNA, Fungal / analysis
  • Dogs
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 28S / analysis*
  • RNA, Ribosomal, 28S / genetics
  • Reagent Kits, Diagnostic
  • Sequence Homology, Nucleic Acid

Substances

  • DNA, Fungal
  • RNA, Ribosomal, 28S
  • Reagent Kits, Diagnostic

Associated data

  • GENBANK/AF378734
  • GENBANK/AF378735
  • GENBANK/AF378736
  • GENBANK/AF378738
  • GENBANK/AF378739
  • GENBANK/AF378740
  • GENBANK/AF448547
  • GENBANK/AF448549
  • GENBANK/AF448550
  • GENBANK/AF448551
  • GENBANK/AF506033
  • GENBANK/AF506034
  • GENBANK/AF506035
  • GENBANK/AF506036