Background & objective: A new drug resistance-related gene fragment which was 494 bp long was found using suppression subtractive hybridization (SSH) and its full-length cDNA fragment was cloned by the authors. This study was designed to determine the expression of this lung cancer drug resistance-related gene (LCDRG) in lung cancer tissues, juxtacancerous tissues, and five lung cancer cell strains.
Methods: The expression of LCDRG was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) method in 38 lung cancer tissues,12 juxtacancerous tissues, and 5 lung cancer cell strains.
Results: The expression of LCDRG in cancer tissues was significantly higher than that in juxtacancerous tissue (P< 0.001). There was no significance of the expression among adenocarcinoma, brochioloalveolar carcinoma, and squamous carcinoma. In lung cancer cell strains, the expression levels of LCDRG in adenocarcinoma cell strains SPC-A-1 and A549, big cell lung cancer cell strain H460, small cell lung cancer cell strains H446 and SH77 were decreased gradually.
Conclusion: LCDRG is closely related to lung cancer and may be involved in the pathogenesis of lung cancer.