Extracellular acidic polysaccharide production by a two-membered bacterial coculture

Biosci Biotechnol Biochem. 2003 Jan;67(1):8-14. doi: 10.1271/bbb.67.8.

Abstract

A two-membered coculture of strains KYM-7 and KYM-8, identified as Cellulomonas cellulans and Agrobacterium tumefaciens, respectively, produced a large amount of an extracellular polysaccharide, designated APK-78, from starch. Each strain in pure culture produced only very little amount of polysaccharide from starch; the coexistence of the two strains from the early stage of cultivation was indispensable for a large amount of polysaccharide to be produced. The polysaccharide APK-78 was acidic and composed of glucose, galactose, succinic acid, and pyruvic acid with a molar ratio of 8.1:1.0:1.7:1.0, indicating that it is a succinoglycan type of polysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agrobacterium tumefaciens / genetics
  • Agrobacterium tumefaciens / metabolism*
  • Carbon / metabolism
  • Cellulomonas / genetics
  • Cellulomonas / metabolism*
  • Chromatography, High Pressure Liquid
  • Colony Count, Microbial
  • Culture Media
  • DNA, Bacterial / biosynthesis
  • Magnetic Resonance Spectroscopy
  • Phylogeny
  • Polysaccharides / biosynthesis*
  • Polysaccharides / isolation & purification
  • Quinones / metabolism
  • Starch

Substances

  • Culture Media
  • DNA, Bacterial
  • Polysaccharides
  • Quinones
  • Carbon
  • Starch