Biosynthesis of Vitamin B(6) in yeast. Incorporation pattern of trioses

J Org Chem. 2003 May 2;68(9):3486-93. doi: 10.1021/jo020730k.

Abstract

The biosynthetic origin of the C(3) unit, C-6,5,5', of pyridoxamine was investigated in two yeasts, Candida utilis ATCC 9256 and Saccharomyces cerevisiae ATCC 7752. The incorporation patterns within pyridoxamine bishydrochloride derived from variously multiply (13)C- and (2)H-labeled samples of glycerol and glyceraldehyde, established by NMR spectroscopy, indicate that the three-carbon unit C-6,5,5' of pyridoxamine is derived intact from a triose.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Candida / metabolism*
  • Carbon Isotopes
  • Deuterium
  • Escherichia coli / metabolism
  • Glyceraldehyde
  • Glycerol
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular
  • Pyridoxamine / biosynthesis*
  • Saccharomyces cerevisiae / metabolism*
  • Trioses / metabolism
  • Vitamin B 6 / biosynthesis*

Substances

  • Carbon Isotopes
  • Trioses
  • Glyceraldehyde
  • Pyridoxamine
  • Vitamin B 6
  • Deuterium
  • Glycerol