Analysis of urinary nucleosides. III. Identification of 5'-deoxycytidine in urine of a patient with head and neck cancer

Edward Dudley; Filip Lemiere; Walter Van Dongen; James I Langridge; Salah El-Sharkawi; David E Games; Eddy L Esmans; Russell P Newton

doi:10.1002/rcm.1034

Analysis of urinary nucleosides. III. Identification of 5'-deoxycytidine in urine of a patient with head and neck cancer

Rapid Commun Mass Spectrom. 2003;17(11):1132-6. doi: 10.1002/rcm.1034.

Authors

Edward Dudley¹, Filip Lemiere, Walter Van Dongen, James I Langridge, Salah El-Sharkawi, David E Games, Eddy L Esmans, Russell P Newton

Affiliation

¹ Biochemistry Group, School of Biological Sciences, Wallace Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK.

PMID: 12772268
DOI: 10.1002/rcm.1034

Abstract

Modified nucleosides in the urine have been postulated to be diagnostic indices of disease, particularly of cancer. The urine of a patient with terminal head and neck cancer has been found to contain a modified nucleoside with a protonated molecule of m/z 228. By means of high-performance liquid chromatography/ion trap mass spectrometry (HPLC/ITMS) and capillary liquid chromatography/triple quadruple mass spectrometry (CapLC/TQMS) we have identified the compound as 5'-deoxycytidine. This is the first report of 5'-deoxycytidine in man: in addition to the elucidation of its structure, its possible origins and the potential significance of its occurrence are discussed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid
Deoxycytidine / urine*
Head and Neck Neoplasms / urine*
Humans
Mass Spectrometry

Substances

Deoxycytidine