Comparative analysis for expressed genes by polymerase chain reaction using module-shuffling primers

C Uematsu; J Nishida; K Okano; H Kambara; F Miura; T Ito; Y Sakaki

doi:10.1093/nass/1.1.91

Comparative analysis for expressed genes by polymerase chain reaction using module-shuffling primers

Nucleic Acids Res Suppl. 2001:(1):91-2. doi: 10.1093/nass/1.1.91.

Authors

C Uematsu¹, J Nishida, K Okano, H Kambara, F Miura, T Ito, Y Sakaki

Affiliation

¹ Biosystems Research Department, Central Research Laboratory, Hitachi Ltd.

PMID: 12836279
DOI: 10.1093/nass/1.1.91

Abstract

We have developed a method for comparative analysis of gene expression. It is based on competitive PCR amplification with module-shuffling primers, followed by gel electrophoresis in a fluorescent DNA sequencer. In this method, tagged-cDNA restriction fragments derived from different sources were amplified in one tube at the same amplification efficiency. The method can detect different amounts of each expressed gene, up to difference in amounts of 30%. The method was successfully used for comparative analysis of expressed genes in yeast.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

DNA Primers*
DNA Shuffling
Gene Expression*
Polymerase Chain Reaction / methods*
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism

Substances

DNA Primers