To study the blood hormone level between diet-induced obesity-resistant (DIO-R) rats and diet-induced obesity(DIO) rats in blood hormone.
Methods: Fifty male Sprague-dawley (SD) rats were randomly divided into control group and high-fat group and they were fed with basic diet and high-fat diet respectively for 13 weeks. DIO-R and DIO rats were selected according to their body weight, then the intake of diet and body fat contents, the level of serum insulin, serum leptin and plasma NPY were determined by radioimmunoassay. The results showed that the weight, diet intake and body fat of DIO-R rats were significantly lower than those of DIO rats(P < 0.05). The level of serum insulin and plasma NPY of DIO-R rats were lower than those of DIO rats(P < 0.05). The level of serum leptin of rats in high-fat diet group was higher than that of the rat in basic diet group, but there was no significant difference between DIO-R and DIO rats(P > 0.05).
Conclusion: High fat diets can induce SD rat to develop obesity and obesity-resistance. The balance of insulin-leptin-NPY feedback may play a partial of role in resisting diet-induced obesity of rats.