Abstract
We have previously shown that mu-selective opioid agonists activate both an inward rectifying and a voltage-gated potassium conductance in acutely dissociated non-pyramidal neurons from rat hippocampus. We now report that the opioid-activated voltage-gated potassium conductance was blocked by the membrane permeable cAMP analogue 8-bromo-cAMP. In contrast, 8-bromo-cGMP failed to inhibit opioid activation of the voltage-gated potassium current. These results suggest that the opioid-activated potassium channel is regulated by cAMP-dependent phosphorylation.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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8-Bromo Cyclic Adenosine Monophosphate / pharmacology*
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Animals
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Cyclic GMP / analogs & derivatives
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Cyclic GMP / pharmacology
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Depression, Chemical
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Endorphins / pharmacology
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Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
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Enkephalins / pharmacology
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Hippocampus / cytology*
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Hippocampus / drug effects
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Ion Channel Gating / drug effects*
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Male
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Membrane Potentials / drug effects
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Neurons / drug effects*
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Neurons / physiology
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Phosphorylation
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Potassium / metabolism*
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Potassium Channels / drug effects*
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Rats
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Rats, Inbred Strains
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Receptors, Opioid / drug effects*
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Receptors, Opioid / physiology
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Receptors, Opioid, mu
Substances
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Endorphins
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Enkephalins
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Potassium Channels
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Receptors, Opioid
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Receptors, Opioid, mu
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Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
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8-Bromo Cyclic Adenosine Monophosphate
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8-bromocyclic GMP
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morphiceptin, N-Me-Phe(3)-
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Cyclic GMP
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Potassium