8-Bromo-cAMP blocks opioid activation of a voltage-gated potassium current in isolated hippocampal neurons

Neurosci Lett. 1992 Mar 16;137(1):137-40. doi: 10.1016/0304-3940(92)90316-y.

Abstract

We have previously shown that mu-selective opioid agonists activate both an inward rectifying and a voltage-gated potassium conductance in acutely dissociated non-pyramidal neurons from rat hippocampus. We now report that the opioid-activated voltage-gated potassium conductance was blocked by the membrane permeable cAMP analogue 8-bromo-cAMP. In contrast, 8-bromo-cGMP failed to inhibit opioid activation of the voltage-gated potassium current. These results suggest that the opioid-activated potassium channel is regulated by cAMP-dependent phosphorylation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 8-Bromo Cyclic Adenosine Monophosphate / pharmacology*
  • Animals
  • Cyclic GMP / analogs & derivatives
  • Cyclic GMP / pharmacology
  • Depression, Chemical
  • Endorphins / pharmacology
  • Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
  • Enkephalins / pharmacology
  • Hippocampus / cytology*
  • Hippocampus / drug effects
  • Ion Channel Gating / drug effects*
  • Male
  • Membrane Potentials / drug effects
  • Neurons / drug effects*
  • Neurons / physiology
  • Phosphorylation
  • Potassium / metabolism*
  • Potassium Channels / drug effects*
  • Rats
  • Rats, Inbred Strains
  • Receptors, Opioid / drug effects*
  • Receptors, Opioid / physiology
  • Receptors, Opioid, mu

Substances

  • Endorphins
  • Enkephalins
  • Potassium Channels
  • Receptors, Opioid
  • Receptors, Opioid, mu
  • Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
  • 8-Bromo Cyclic Adenosine Monophosphate
  • 8-bromocyclic GMP
  • morphiceptin, N-Me-Phe(3)-
  • Cyclic GMP
  • Potassium