Abstract
Sulfate activating enzymes, ATP sulfurylase and APS kinase, were newly isolated from a thermophile, Bacillus stearothermophilus. Adenosine 3'-phosphate 5'-phosphosulfate (PAPS) was synthesized by these enzymes. The reaction proceeded more efficiently when an ATP-regeneration system, using acetate kinase, was coupled to the reaction system.
MeSH terms
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Acetate Kinase / chemistry
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Adenosine Triphosphate / chemistry*
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Geobacillus stearothermophilus / enzymology
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Phosphoadenosine Phosphosulfate / chemical synthesis*
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Phosphoadenosine Phosphosulfate / isolation & purification
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Phosphotransferases (Alcohol Group Acceptor)*
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Phosphotransferases / chemistry
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Sulfate Adenylyltransferase / chemistry
Substances
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Phosphoadenosine Phosphosulfate
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Adenosine Triphosphate
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Phosphotransferases
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Phosphotransferases (Alcohol Group Acceptor)
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adenylylsulfate kinase
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Acetate Kinase
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Sulfate Adenylyltransferase