A simplified cytomegalovirus pp65 antigenemia assay procedure

J Clin Virol. 2003 Dec;28(3):317-22. doi: 10.1016/s1386-6532(03)00075-1.

Abstract

A simplified cytomegalovirus (CMV) pp65 antigenemia assay using a one-step erythrocyte lysis, fixation and permeabilization process was compared with a standard protocol, the CMV CINAkit (Argene Biosoft) assay. The results were comparable, both quantitatively and qualitatively. The new method saves time. It also provides flexibility because the cell suspension can be stored so that test completion can be deferred if so desired.

Publication types

  • Evaluation Study

MeSH terms

  • Cytomegalovirus / isolation & purification*
  • Cytomegalovirus Infections / diagnosis*
  • Cytomegalovirus Infections / virology
  • Fluorescent Antibody Technique
  • Humans
  • Leukocytes / virology*
  • Phosphoproteins / blood*
  • Reagent Kits, Diagnostic
  • Time Factors
  • Viral Matrix Proteins / blood*
  • Viremia / diagnosis*
  • Viremia / virology

Substances

  • Phosphoproteins
  • Reagent Kits, Diagnostic
  • Viral Matrix Proteins
  • cytomegalovirus matrix protein 65kDa