Abstract
A four-color set of negatively charged, single dye as well as energy transfer dye labeled-ddNTPs were synthesized and evaluated in combination with a novel polymerase in a "direct-load" DNA sequencing, obviating the laborious and time consuming post-reaction work-up.
MeSH terms
-
Base Sequence
-
Coloring Agents*
-
DNA / chemistry*
-
Deoxyribonucleotides / chemical synthesis*
-
Dideoxynucleosides / chemical synthesis*
-
Energy Transfer
-
Indicators and Reagents
Substances
-
Coloring Agents
-
Deoxyribonucleotides
-
Dideoxynucleosides
-
Indicators and Reagents
-
DNA