Negatively charged, dye labeled-dideoxynucleotides for "direct-load" DNA sequencing

Nucleosides Nucleotides Nucleic Acids. 2003 May-Aug;22(5-8):1471-4. doi: 10.1081/NCN-120023013.

Abstract

A four-color set of negatively charged, single dye as well as energy transfer dye labeled-ddNTPs were synthesized and evaluated in combination with a novel polymerase in a "direct-load" DNA sequencing, obviating the laborious and time consuming post-reaction work-up.

MeSH terms

  • Base Sequence
  • Coloring Agents*
  • DNA / chemistry*
  • Deoxyribonucleotides / chemical synthesis*
  • Dideoxynucleosides / chemical synthesis*
  • Energy Transfer
  • Indicators and Reagents

Substances

  • Coloring Agents
  • Deoxyribonucleotides
  • Dideoxynucleosides
  • Indicators and Reagents
  • DNA