Cloning and mutation analysis of ZFP276 as a candidate tumor suppressor in breast cancer

J Hum Genet. 2003;48(12):668-671. doi: 10.1007/s10038-003-0088-1. Epub 2003 Nov 7.

Abstract

Loss of heterozygosity (LOH) involving chromosome 16q23.4 occurs frequently in breast tumors, which suggests that this region may contain a tumor suppressor gene. Since ZFP276 is located in this region, we have therefore cloned and performed mutation analysis of its coding region in 70 breast tumors. One silent polymorphism and two alterations predicted to result in amino acid changes were detected. Absence of the wild-type allele in tumors carrying the E530D variant suggests a possible role for this change in tumorigenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Blotting, Northern
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Chromosomes, Human, Pair 16
  • Cloning, Molecular
  • DNA Mutational Analysis
  • DNA, Complementary / metabolism
  • Female
  • Genes, Tumor Suppressor*
  • Humans
  • Models, Genetic
  • Molecular Sequence Data
  • Nuclear Proteins
  • Polymorphism, Genetic*
  • Polymorphism, Single-Stranded Conformational
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Tumor Suppressor Proteins / genetics*
  • Zinc Fingers

Substances

  • DNA, Complementary
  • Nuclear Proteins
  • RNA, Messenger
  • Tumor Suppressor Proteins
  • ZNF276 protein, human