Abstract
The role of cyclooxygenase (COX)-1 and -2 in prostanoid formation and modulation of pressor responses to ANG II was investigated in the pulmonary and systemic vascular beds in the rat. In the present study, selective COX-1 and -2 inhibitors attenuated increases in pulmonary arterial pressure and decreases in systemic arterial pressure in response to arachidonic acid but did not alter responses to PGE1 or U-46619. The selective COX-1 and -2 inhibitors did not modify systemic pressor responses to injections or infusions of ANG II or pulmonary pressor responses to injections of the peptide. COX-2 inhibitors did not alter, whereas a COX-1 inhibitor depressed, arachidonic acid-induced platelet aggregation. These data provide evidence in support of the hypothesis that prostanoid synthesis occurs by way of the COX-1 and -2 pathways in the pulmonary and systemic vascular beds but that pressor responses to ANG II are not mediated or modulated by these pathways in the rat.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / pharmacology
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Alprostadil / pharmacology
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Angiotensin II / pharmacology*
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Animals
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Arachidonic Acid / pharmacology
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Blood Pressure / drug effects
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Blood Pressure / physiology
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Cardiac Catheterization / methods
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Cyclooxygenase 1
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Cyclooxygenase 2
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Cyclooxygenase 2 Inhibitors
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Cyclooxygenase Inhibitors / pharmacology
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Isoenzymes / metabolism*
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Membrane Proteins
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Nitrobenzenes / pharmacology
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Platelet Aggregation / drug effects
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Platelet Aggregation / physiology
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Prostaglandin-Endoperoxide Synthases / metabolism*
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Prostaglandins / biosynthesis*
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Pulmonary Circulation / drug effects
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Pulmonary Circulation / physiology*
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Pyrazoles / pharmacology
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Rats
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Rats, Sprague-Dawley
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Sulfonamides / pharmacology
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Vasoconstrictor Agents / pharmacology*
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Vasodilator Agents / pharmacology
Substances
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4-(5-(4-chlorophenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl)benzenesulfonamide
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Cyclooxygenase 2 Inhibitors
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Cyclooxygenase Inhibitors
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Isoenzymes
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Membrane Proteins
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Nitrobenzenes
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Prostaglandins
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Pyrazoles
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SC 560
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Sulfonamides
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Vasoconstrictor Agents
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Vasodilator Agents
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Angiotensin II
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N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
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Arachidonic Acid
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15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
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Cyclooxygenase 1
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Cyclooxygenase 2
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Prostaglandin-Endoperoxide Synthases
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Ptgs1 protein, rat
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Alprostadil