Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6

J Vet Sci. 2001 Aug;2(2):115-20.

Abstract

This paper describes the cloning and sequence analysis of the cDNAs encoding the canine homologues of interleukin-3 (IL-3) and interleukin-6 (IL-6). The coding sequences for canine IL-3 and IL-6 were obtained by using the reverse transcription polymerase chain reaction (RT-PCR) with RNA harvested from canine peripheral blood mononuclear cells (PBMCs). Canine IL-3 cDNA includes a single open reading frame of 432 nucleotides, which encodes a 143 amino acid polypeptide and has 44.7, 42.4, 37 and 23.7% homology with the cow, sheep, human and rat IL-3 sequences, respectively. Canine IL-6 cDNA (GenBank accession number; AF275796) encodes a putative 20-amino acid signal peptide followed by a 187-amino acid mature protein. The predicted amino acid sequence of canine IL-6 shares 60.4, 77.2, 71.0, 55.8 and 42.0% sequence identity with those of human, feline, porcine, sheep and rat IL-6, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Concanavalin A / pharmacology
  • DNA, Complementary / chemistry*
  • Dogs / blood
  • Dogs / genetics
  • Dogs / immunology*
  • Interleukin-3 / chemistry
  • Interleukin-3 / genetics*
  • Interleukin-6 / chemistry
  • Interleukin-6 / genetics*
  • Leukocytes, Mononuclear / chemistry
  • Leukocytes, Mononuclear / drug effects
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Protein Sorting Signals / genetics
  • RNA / blood
  • RNA / genetics
  • Reverse Transcriptase Polymerase Chain Reaction / veterinary
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid

Substances

  • DNA, Complementary
  • Interleukin-3
  • Interleukin-6
  • Protein Sorting Signals
  • Concanavalin A
  • RNA

Associated data

  • GENBANK/AF275796