Abstract
Fibroblast growth factor-binding protein (FGF-BP) releases immobilized FGFs from the extracellular matrix and can function as an angiogenic switch molecule in cancer. Here we show that FGF-BP is up-regulated in early dysplastic lesions of the human colon that are typically associated with a loss of adenomatous polyposis coli and up-regulation of beta-catenin. In addition, FGF-BP expression is induced in dysplastic lesions in ApcMin/+ mice in parallel with the up-regulation of beta-catenin. Also, in cell culture studies FGF-BP is induced by beta-catenin through direct activation of the FGF-BP gene promoter. We conclude that FGF-BP is a target gene of beta-catenin.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Adenoma / genetics
-
Adenoma / metabolism
-
Animals
-
Carrier Proteins / biosynthesis*
-
Carrier Proteins / genetics
-
Colonic Neoplasms / genetics
-
Colonic Neoplasms / metabolism*
-
Cytoskeletal Proteins / biosynthesis
-
Cytoskeletal Proteins / genetics
-
Cytoskeletal Proteins / physiology*
-
Gene Expression Regulation, Neoplastic / drug effects
-
Humans
-
Intercellular Signaling Peptides and Proteins
-
Intracellular Signaling Peptides and Proteins
-
Lithium Chloride / pharmacology
-
Mice
-
Mice, Inbred C57BL
-
Promoter Regions, Genetic
-
RNA, Messenger / biosynthesis
-
RNA, Messenger / genetics
-
Trans-Activators / biosynthesis
-
Trans-Activators / genetics
-
Trans-Activators / physiology*
-
Transfection
-
Up-Regulation / drug effects
-
beta Catenin
Substances
-
CTNNB1 protein, human
-
CTNNB1 protein, mouse
-
Carrier Proteins
-
Cytoskeletal Proteins
-
Fgfbp1 protein, mouse
-
Intercellular Signaling Peptides and Proteins
-
Intracellular Signaling Peptides and Proteins
-
RNA, Messenger
-
Trans-Activators
-
beta Catenin
-
FGFBP1 protein, human
-
Lithium Chloride