Subcellular localization of MAK-V/Hunk protein kinase expressed in COS-1 cells

Cell Biol Int. 2004;28(1):49-56. doi: 10.1016/j.cellbi.2003.10.002.

Abstract

MAK-V/Hunk is a MARK/Par-1-related protein kinase, whose function is unknown. We studied the subcellular localization of MAK-V/Hunk in COS-1 cells by immunofluorescence. It has a nucleocytoplasmic distribution and is localized to the centrosome, as indicated by co-localization with gamma-tubulin. A putative kinase-deficient mutant, with a mutation in the invariant lysine residue in the catalytic domain, was not targeted to the nucleus or centrosome. These results suggest that the nuclear and centrosomal targeting of MAK-V/Hunk is specific, and is likely to be coupled to its catalytic activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Amino Acid Substitution
  • Animals
  • Antibodies, Monoclonal
  • COS Cells
  • Catalytic Domain
  • Cell Line
  • Centrosome / chemistry
  • Centrosome / enzymology
  • Chlorocebus aethiops
  • Fluorescent Antibody Technique
  • Gene Expression
  • Mice
  • Plasmids
  • Protein Kinases / analysis*
  • Protein Kinases / genetics
  • Protein Kinases / metabolism
  • Protein Serine-Threonine Kinases

Substances

  • Antibodies, Monoclonal
  • Protein Kinases
  • Hunk protein, mouse
  • Protein Serine-Threonine Kinases