To assess the diagnostic accuracy of ex vivo coronary artery arteriography, 12 sheep hearts were harvested, after random multiple coronary occlusions were created. The hearts were radiographed either in the flat (n = 6) or upright suspended (n = 6) position with the aorta cross-clamped. The aortic root was cannulated with an 18-gauge needle and flushed with cold normal saline solution. Five milliliters of iodinated contrast media was injected under constant hand pressure, and angiographic views were taken with a conventional, portable x-ray machine. The aortic root was then immediately flushed with normal saline solution under constant pressure to minimize contrast contact with the vascular endothelium. Total elapsed time including film development was 7 to 10 minutes. In hearts that were radiographed lying flat, a ventriculogram was obtained because of aortic incompetence. In hearts suspended in the upright position, normal coronary anatomy and previously placed coronary occlusions were easily detected in every heart that was read blindly by angiographers. To assess the effects of the contrast media on ventricular performance, five rabbit hearts were harvested and mounted on a Langendorff apparatus for functional analysis at controlled left ventricle volumes. Diastolic and systolic left ventricular pressures were unaffected by the dye injection. This simple and rapid method to evaluate coronary anatomy of the donor heart could be potentially useful in the assessment of older donor hearts in the operating room, thus increasing the volume of donor hearts available for transplantation.