Electrophoresis time impacts the denaturing gradient gel electrophoresis-based assessment of bacterial community structure

W V Sigler; C Miniaci; J Zeyer

doi:10.1016/j.mimet.2003.11.011

Electrophoresis time impacts the denaturing gradient gel electrophoresis-based assessment of bacterial community structure

J Microbiol Methods. 2004 Apr;57(1):17-22. doi: 10.1016/j.mimet.2003.11.011.

Authors

W V Sigler¹, C Miniaci, J Zeyer

Affiliation

¹ Department of Earth, Ecological and Environmental Sciences, University of Toledo, 2801 W. Bancroft MS 604, Toledo, OH 43606, USA. von.sigler@utoledo.edu

PMID: 15003684
DOI: 10.1016/j.mimet.2003.11.011

Abstract

We investigated the impact of denaturing gradient gel electrophoresis (DGGE) run time on the assessment of bacterial community structure. Results indicated that increased electrophoresis run time (while maintaining 1000 volt-hours) resulted in dissimilar profiles, likely due to instability of the denaturing gradient. We recommend that DGGE run times be minimized to provide optimal band resolution, as extended electrophoresis times can greatly impact subsequent band-based analyses.

MeSH terms

Electrophoresis, Polyacrylamide Gel / methods*
Nucleic Acid Denaturation / drug effects
Soil Microbiology*
Time Factors