Abstract
We generated the small interference RNAs to specifically silence the expression of neural salient serine/arginine rich protein 1 (NSSR1) and showed that the inhibition of NSSR1 expression in mouse embryonic carcinoma cells (P19) reduces neuronal differentiation. By contrast, its over-expression promotes the differentiation. Neither inhibition nor over-expression shows distinct effect on cell proliferation. The over-expression increases the inclusion of NCAM L1 exon2 while the inhibition reduces the inclusion. The splicing of kinase insert free isoform of TrkC (TrkC-K1) is increased by the over-expression. The results demonstrate that NSSR1 promotes neuronal differentiation and the splicing of NCAML1 exon2 and TrkC-K1.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing
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Animals
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Blotting, Northern / methods
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Blotting, Western / methods
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Bromodeoxyuridine / metabolism
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Carcinoma
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Carrier Proteins / physiology*
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Cell Count
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Cell Cycle Proteins
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Cell Differentiation / physiology*
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Cell Line, Tumor
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Embryo, Mammalian
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Exons
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Gene Expression / drug effects
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Gene Expression Regulation
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Mice
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Neoplasm Proteins / physiology*
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Neural Cell Adhesion Molecules / genetics
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Neural Cell Adhesion Molecules / metabolism
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Neurons / pathology*
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RNA, Messenger / biosynthesis
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RNA, Small Interfering / pharmacology
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RNA-Binding Proteins / physiology*
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Receptor, trkC / genetics
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Receptor, trkC / metabolism
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Receptors, GABA-A / genetics
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Receptors, GABA-A / metabolism
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Repressor Proteins / physiology*
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Transfection / methods
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Tubulin / metabolism
Substances
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Carrier Proteins
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Cell Cycle Proteins
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Fusip1 protein, mouse
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Neoplasm Proteins
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Neural Cell Adhesion Molecules
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RNA, Messenger
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RNA, Small Interfering
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RNA-Binding Proteins
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Receptors, GABA-A
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Repressor Proteins
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Tubulin
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Receptor, trkC
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Bromodeoxyuridine