Abstract
Vesicular glutamate transporters (VGLUTs) 1 and 2 show a mutually exclusive distribution in the adult brain that suggests specialization for synapses with different properties of release. Consistent with this distribution, inactivation of the VGLUT1 gene silenced a subset of excitatory neurons in the adult. However, the same cell populations exhibited VGLUT1-independent transmission early in life. Developing hippocampal neurons transiently coexpressed VGLUT2 and VGLUT1 at distinct synaptic sites with different short-term plasticity. The loss of VGLUT1 also reduced the reserve pool of synaptic vesicles. Thus, VGLUT1 plays an unanticipated role in membrane trafficking at the nerve terminal.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Animals, Newborn
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Brain / cytology
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Brain / metabolism*
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Membrane / physiology
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Cells, Cultured
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Cerebellum / metabolism
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Cerebellum / ultrastructure
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Excitatory Postsynaptic Potentials
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Glutamic Acid / metabolism
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Hippocampus / cytology
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Hippocampus / metabolism
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Hippocampus / ultrastructure
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In Situ Hybridization
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Membrane Transport Proteins*
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Mice
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Mice, Knockout
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Nerve Tissue Proteins / metabolism
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Neurons / metabolism*
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Neurons / physiology
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Patch-Clamp Techniques
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Purkinje Cells / physiology
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Pyramidal Cells / metabolism
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Synapses / metabolism*
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Synapses / ultrastructure
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Synaptic Transmission*
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Synaptic Vesicles / metabolism*
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Synaptic Vesicles / physiology
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Vesicular Glutamate Transport Protein 1
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Vesicular Glutamate Transport Protein 2
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Vesicular Transport Proteins*
Substances
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Carrier Proteins
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Membrane Transport Proteins
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Nerve Tissue Proteins
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Slc17a6 protein, mouse
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Slc17a7 protein, mouse
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Vesicular Glutamate Transport Protein 1
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Vesicular Glutamate Transport Protein 2
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Vesicular Transport Proteins
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Glutamic Acid