The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens

Sumeth Termmathurapoj; Saovanee Leelayoova; Pote Aimpun; Umaporn Thathaisong; Thirayost Nimmanon; Paanjit Taamasri; Mathirut Mungthin

doi:10.1007/s00436-004-1157-x

The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens

Parasitol Res. 2004 Aug;93(6):445-7. doi: 10.1007/s00436-004-1157-x. Epub 2004 Jul 9.

Authors

Sumeth Termmathurapoj¹, Saovanee Leelayoova, Pote Aimpun, Umaporn Thathaisong, Thirayost Nimmanon, Paanjit Taamasri, Mathirut Mungthin

Affiliation

¹ Department of Pathology, Phramongkutklao College of Medicine, 315 Ratchawithi Road, Ratchathewi, 10400, Bangkok, Thailand.

PMID: 15243800
DOI: 10.1007/s00436-004-1157-x

Abstract

When in vitro cultivation was used as the 'gold standard' for the detection of Blastocystis hominis in stool specimens, simple smear and trichrome staining showed sensitivities of 16.7% and 40.2% and specificities of 94% and 80.4%, respectively. In vitro cultivation also enhanced PCR amplification for the detection of B. hominis in stool specimens. Our data show the usefulness of in vitro cultivation for the detection and molecular study of B. hominis in stool specimens.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Blastocystis Infections / diagnosis*
Blastocystis Infections / parasitology*
Blastocystis hominis / genetics
Blastocystis hominis / growth & development
Blastocystis hominis / isolation & purification*
DNA, Protozoan / genetics
DNA, Protozoan / isolation & purification
Feces / parasitology
Humans
In Vitro Techniques
Polymerase Chain Reaction
Staining and Labeling

Substances

DNA, Protozoan