Phenylpropanoid NF-kappaB inhibitors from Bupleurum fruticosum

Planta Med. 2004 Oct;70(10):914-8. doi: 10.1055/s-2004-832616.

Abstract

Two phenylpropanoids from Bupleurum fruticosum (Apiaceae/Umbelliferae) were shown to inhibit the transcriptional activity induced by PMA or TNFalpha of an NF-kappaB-controlled reporter gene. Western blot experiments indicated that the phenylpropanoids did not prevent IkappaBalpha degradation, suggesting that their molecular target is at a post-IKB degradation level. Both compounds prevented cytokine (IL-1, IL-6, TNF, IL-8) release and prostaglandin E2 synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apiaceae*
  • Blotting, Western
  • Dose-Response Relationship, Drug
  • Enzyme Activation / drug effects
  • HeLa Cells / drug effects
  • Humans
  • Interleukin-6 / genetics
  • Luciferases / genetics
  • NF-kappa B / antagonists & inhibitors
  • NF-kappa B / metabolism*
  • Phytotherapy*
  • Plant Components, Aerial
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Transcription, Genetic / drug effects
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Interleukin-6
  • NF-kappa B
  • Plant Extracts
  • Tumor Necrosis Factor-alpha
  • Luciferases