Abstract
Two phenylpropanoids from Bupleurum fruticosum (Apiaceae/Umbelliferae) were shown to inhibit the transcriptional activity induced by PMA or TNFalpha of an NF-kappaB-controlled reporter gene. Western blot experiments indicated that the phenylpropanoids did not prevent IkappaBalpha degradation, suggesting that their molecular target is at a post-IKB degradation level. Both compounds prevented cytokine (IL-1, IL-6, TNF, IL-8) release and prostaglandin E2 synthesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apiaceae*
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Blotting, Western
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Dose-Response Relationship, Drug
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Enzyme Activation / drug effects
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HeLa Cells / drug effects
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Humans
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Interleukin-6 / genetics
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Luciferases / genetics
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NF-kappa B / antagonists & inhibitors
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NF-kappa B / metabolism*
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Phytotherapy*
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Plant Components, Aerial
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Plant Extracts / administration & dosage
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Plant Extracts / pharmacology*
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Plant Extracts / therapeutic use
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Transcription, Genetic / drug effects
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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Interleukin-6
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NF-kappa B
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Plant Extracts
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Tumor Necrosis Factor-alpha
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Luciferases